Genetic diversity is our heritage, efficient germplasm collection and its evaluation is the key factor to address the problem and to identify the wealth of the important germplasm growing in our close vicinity. In the present study a total of 40 genotypes of Phaseolus vulgaris (L) were collected from plane and mountainous areas of Khyber Pakhtunkhwa Pakistan, and investigated for morphological and biochemical genetic diversity. An appreciable variation was observed for seed colour and 100 seed weight. SDS-PAGE was carried out and 37% genetic disagreement was observed in the collected lines with percent disagreement ranging from 0.00 0.71. To fractionate and explain the level of genetic diversity, the electropherogram was further divided into three zones and showing 37%, 36 % and 39% genetic disagreement respectively and subsequently analyzed through cluster analysis (how to use computer software for cluster analysis see tutorial for cluster analysis narrated in this book). Cluster analysis exhibited moderate level of genetic diversity; to explore the gene pool of Pakistani common bean more collection is required for genetic improvement.