AHA-BUCH

CHARACTERIZING OSTEOBLAST MINERALIZATION USING FTIR

EFFECT OF SIMVASTATIN AND SIMULATED MICROGRAVITY ON OSTEOBLAST MINERALIZATION USING FOURIER TRANSFORM INFRARED SPECTROSCOPY
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ISBN-13:
9783639253030
Einband:
Paperback
Seiten:
116
Autor:
Alka Potdar
Gewicht:
189 g
Format:
220x150x7 mm
Sprache:
Englisch
Beschreibung:

The ability of a cholesterol-reducing drug, simvastatin, to stimulate the formation of hydroxyapatite in an osteoblast-like cell line, was studied using Fourier transform infrared spectroscopy. Hydroxyapatite was detected using both Fourier transform infrared spectroscopy and von Kossa staining techniques a week earlier when cells were cultured in growth media supplemented with simvastatin in a tissue culture polystyrene system. Analysis of infrared spectra using area integration and curve-fitting techniques was performed to quantify the hydroxyapatite content in cells relative to the biological matrix. Infrared spectroscopy was used to quantify the mineralization process. Cells grown in a rotating bioreactor mineralized more rapidly than cells grown in tissue culture polystyrene systems.