Coronavirus Replication and Reverse Genetics

Coronaviruses are the RNA viruses with the largest genome known to date (27 to 32 kb). Members of this virus family affect most domestic animal species, causing important socio-economical losses, and also infect humans. Human coronaviruses were known to cause the winter common cold, a mild infection without important pathological consequences except in immuno-compromised patients. Recently, two new human coronaviruses have emerged, one causing the Severe and Acute Respiratory Syndrome (SARS) that infected more than 8000 individuals, leading to more than 800 deaths in 32 countries. This epidemic mobilized the World Health Organization, which launched travel restrictions to certain parts of the world for the first time in the last 50 years. The fact that coronaviruses, as many other viruses, crossed the species barrier to infect humans has posed a serious challenge to scientists involved in animal and human health. Control of coronavirus-induced diseases can only be the consequence of research on virus molecular biology and pathogenesis. This book contains information on virus genome structure, mechanism of replication and transcription, and the development of tools that make possible reverse genetic studies to understand virus-host interactions and the molecular basis of virus pathogenesis. The book also provides essential information for the development of classical and recombinant vaccines to control coronavirus infections.

"Until the late 1960s, the coronaviruses were not recognized as pathogens responsible for human diseases (common cold). However, in 2003 human coronaviruses (HCoVs) came to worldwide attention with the emergence of the Severe and Acute Respiratory Syndrome (SARS), produced by a coronavirus (SARS-CoV) that infected more than 8000 people, killing about ten percent of them in 32 countries. The increase in research on coronaviruses soon led to the discovery of another human coronavirus (HCoV-NL63) which is prevalent in 7% of hospital patients and has been associated with bronchiolitis and, possibly, conjunctivitis. TOC:Coronavirus genome structure and replication.- Coronavirus transcription: a perspective.- The coronavirus replicase.- Viral and cellular proteins involved in coronavirus replication.- Coronavirus reverse genetics by targeted RNA recombination.- Coronavirus reverse genetics and development of vectors for gene expression.- Reverse genetics of coronaviruses using vaccinia virus vectors.- Development of mouse hepatitis virus and SARS-CoV infectious cDNA constructs.- Subject index."

Coronavirus Genome Structure and Replication.- Coronavirus Transcription: A Perspective.- The Coronavirus Replicase.- Viral and Cellular Proteins Involved in Coronavirus Replication.- Coronavirus Reverse Genetics by Targeted RNA Recombination.- Coronavirus Reverse Genetics and Development of Vectors for Gene Expression.- Reverse Genetics of Coronaviruses Using Vaccinia Virus Vectors.- Development of Mouse Hepatitis Virus and SARS-CoV Infectious cDNA Constructs.