Immunological Tolerance

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.

Provides up-to-date reviews of the literature on essential topics in immunological tolerance

Cell Types Contributing to Immunological Tolerance.- Frontiers of Immunological Tolerance.- Balancing Tolerance and Immunity.- Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow.- Genetic Modification of Dendritic Cells Through the Directed Differentiation of Embryonic Stem Cells.- Generation of Immunocompetent T Cells from Embryonic Stem Cells.- Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells.- Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines.- The Study of Immunological Tolerance In Vitro.- Thymus Organogenesis and Development of the Thymic Stroma.- Generation of a Tissue-Engineered Thymic Organoid.- Studying T-Cell Repertoire Selection Using Fetal Thymus Organ Culture.- Investigating Central Tolerance With Reaggregate Thymus Organ Cultures.- Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles.- Gene Expression Profiling of Dendritic Cells by Microarray.- SAGE Analysis of Cell Types Involved in Tolerance Induction.- Analyzing the Physicodynamics of Immune Cells in a Three-Dimensional Collagen Matrix.- The Study of Immunological Tolerance In Vivo.- Etiology of Autoimmmune Disease.- Animal Models of Spontaneous Autoimmune Disease.- Antigen-Based Therapy and Immune-Regulation in Experimental Autoimmune Encephalomyelitis.- Induction and Regulation of Inflammatory Bowel Disease in Immunodeficient Mice by Distinct CD4+ T-Cell Subsets.- In Vivo Models for the Study of Transplantation Tolerance.- Ectopic Transplantation of Tissues Under the Kidney Capsule.- Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node.- Tracing Tolerance and Immunity In Vivo by CFSE-Labeling of Administered Cells.- Methods for Inducing and Breaking Immunological Tolerance.- Thymic Involution.- Inducing Mixed Chimerism and Transplantation Tolerance Through Allogeneic Bone Marrow Transplantation With Costimulation Blockade.- Induction of Dominant Tolerance Using Monoclonal Antibodies.- Induction of Tolerance by Adoptive Transfer of Treg Cells.- Modulation of the Immune Response Using Dendritic Cell-Derived Exosomes.- Breaking Self-Tolerance to Tumor-Associated Antigens by In Vivo Manipulation of Dendritic Cells.