Immunofluorescence in Clinical Immunology

Immunofluorescence, a suitable laboratory method for themicroscopic demonstration of antigens and antibodies in biologicalmaterials, useable, for example, to provide evidence for thepathogenesis of disease in histological or cytological preparations andfor tumour cell differentiation. For this reason immunofluorescence hasa decisive role as the method of choice for the diagnosis of auto-immunediseases. This primer on immunofluorescence techniques, which firstappeared in 1979, is a richly illustrated handbook suitable for everydaypractical work in the laboratory, useable as both an introduction to thesubject as well as an atlas. In hardly any other area of medicine arethere so many new findings to report. The second edition of this book isconcerned not only with the detection methods which now form anessential and established part of diagnostic techniques, but also withthe most recent research results such as the discovery of antibodiesagainst Auerbach's plexus and against podocytes...

This primer on immunofluorescence techniques, whichfirst appeared in 1979, is a richly illustrated handbook suitable foreveryday practical work in the laboratory, able to be used as both anintroduction to the subject as well as an atlas. In hardly any otherarea of medicine are there so many new findings to report. The secondedition of this book is concerned not only with the detection methodswhich now form an essential and established part of diagnostictechniques, but also with the most recent research results such as thediscovery of antibodies against Auerbach's plexus and againstpodocytes...

1 Introduction to the basic principles and techniques of immunofluor-escence.- 2 Production of antibodies and labelling with fluorochromes.- 3 Characterisation and quality control of antisera and conjugates.- 4 Actual optical immunofluorescence techniques.- 5 Instrumental technique and the documentation of findings.- 6 General advice.- 7 Applications of immunofluorescence.

This primer on immunofluorescence techniques, which first appeared in 1979, is a richly illustrated handbook suitable for everyday practical work in the laboratory, able to be used as both an introduction to the subject as well as an atlas. In hardly any other area of medicine are there so many new findings to report. The second edition of this book is concerned not only with the detection methods which now form an essential and established part of diagnostic techniques, but also with the most recent research results such as the discovery of antibodies against Auerbach's plexus and against podocytes...