MRSA Protocols for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose and then goes into detailed protocols for every step of analysis. Several chapters also include a section with tips on individual steps not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting.
Among the topics discussed in this volume are Staphylococcus aureus infections, MIC analysis, ITS-PCR identification, SCCmec analysis, and Biofilm formation. The book also contains helpful case studies, and authors provide insights into the control, prevention, and treatment of infections.
Oriented towards routine clinical diagnosis, research, and actual practice for treatment of patients infected by MRSA, MRSA Protocols for Methods in Molecular Biology will make a useful basis for anyone interested in instituting a new method for studying MRSA.
Chapters include useful tips for methods and protocols in working with MRSAThe most up-to-date techniques for the detection and investigation of MRSAA comprehensive starting point for those looking for new methods of studying MRSAThorough, thoughtful protocols for every step of analysis
Clinical, Epidemiological, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus (MRSA) Infections.- MRSA Case Studies.- Minimum Inhibitory Concentration (MIC) Analysis and Susceptibility Testing of MRSA.- Internal Transcribed Spacer (ITS)-PCR Identification of MRSA.- Pulsed-Field Gel Electrophoresis of MRSA.- Multilocus Sequence Typing (MLST) of Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Targeted Gene Disruption for the Analysis of Virulence of Staphylococcus aureus 1.- Molecular Analysis of Staphylococcal Superantigens.- Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus.- Comparative Analysis of Staphylococcal Adhesion and Internalization by Epithelial Cells.- Comparative Analysis of MRSA.- Genomic Analysis of Gene Expression of Staphylococcus aureus.- Proteomic Approach to Investigate MRSA.- Environmental Surveillance for MRSA.- Control and Prevention of MRSA Infections.- Treatment of Infections Caused by Resistant Staphylococcus aureus.
