Misbehaving Proteins

An introductory chapter for readers new to the protein folding fieldThorough and clear discussion of computational approaches to understanding and modeling protein aggregationDescriptions of conventional and emerging experimental techniques for analyzing protein misfolding and aggregationCase studies from both academic and industrial researchers, tackling current problems in protein misfolding, aggregation, and stability

Protein Folding, Misfolding, Stability, and Aggregation.- Mathematical Models and Computational Methods.- Nonnative Protein Aggregation.- Simulations of Protein Aggregation.- Experimental Methods.- Elucidating Structure, Stability, and Conformational Distributions during Protein Aggregation with Hydrogen Exchange and Mass Spectrometry.- Application of Spectroscopic and Calorimetric Techniques in Protein Formulation Development.- Small-Angle Neutron Scattering as a Probe for Protein Aggregation at Many Length Scales.- Laser Light Scattering as an Indispensable Tool for Probing Protein Aggregation.- X-ray Diffraction for Characterizing Structure in Protein Aggregates.- Glass Dynamics and the Preservation of Proteins.- Fundamental Studies in Model Systems.- Folding and Misfolding as a Function of Polypeptide Chain Elongation.- Determinants of Protein Folding and Aggregation in P22 Tailspike Protein.- Factors Affecting the Fibrillation of ?-Synuclein, a Natively Unfolded Protein.- Molten Globule-Lipid Bilayer Interactions and Their Implications for Protein Transport and Aggregation.- Protein Product Development.- Self-Association of Therapeutic Proteins.- Mutational Approach to Improve Physical Stability of Protein Therapeutics Susceptible to Aggregation.