Advanced Chromatographic and Electromigration Methods in Biosciences

This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.

Deals with chromatographic and electrophoretic methods applied for the separation of biologically relevant compounds. This book also deals with individual separation modes to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.

Preface. List of Contributors. 1. Selection of chromatographic methods for biological materials (T. Hanai). 2. Strategies for electromigration separations of biological macromolecules (H. Arakawa, M. Maeda, T. Hanai). 3. Sample preparation (Q. Yang, A.J. Tomlinson, S. Naylor). 4. Selected derivatization reactions (Z. Deyl, I. Mikšík, E. Tesařová). 5. Enantioselective separations (E. Tesařová, D.W. Armstrong). 6. Carbohydrates, polymeric sugars and their constituents (M. Estrella Legaz, M.M. Pedrosa, C. Vicente). 7. Carboxylic acids (Z. Deyl, I. Mikšík). 8. Herbal organic acids (S.Z. Yao et al.). 9. Analysis of lipids by thin-layer chromatography (N.C. Shantha, G.E. Napolitano). 10. Biogenic amines (Z. Deyl, I. Mikšík). 11. Amino acids (I. Molnár-Perl). 12. Peptides (Z. Deyl, I. Mikšík). 13. Protein analysis (J.F. Banks). 14. Nucleic acids and their constituents (M. Gilar, D.L. Smisek, A.S. Cohen). 15. Compounds containing heterocyclic nitrogen, oxygen and sulfur (I. Mikšík, Z. Deyl). 16. Vitamins (A. Rizzolo, S. Polesello). 17. Antibiotics (S.A. Barker). 18. Pesticides in environmental samples (K. Fodor-Csorba). 19. Monitoring of process impurities in drugs (S. Husain, R. Nageswara Rao). 20. Procedures in hospital laboratories (R.M. Dorizzi). 21. Toxicological and forensic applications (F. Tagliaro et al.). 22. Inorganic analysis and speciation (A.R. Timerbaev, W. Buchberger). 23. Common pitfalls in CE and HPLC (M. Arellano, F. Couderc). Index of Compounds Separated.